HPLC Advance Course

HPLC Advance Course

The HPLC Advance Course contains the following modules:

  • Introduction to HPLC analysis.
  • GLP and GDP related to HPLC analysis
  • Type of Quantitative and Qualitative analysis performed on HPLC
    • Identification
    • Assay
    • Related substances
    • Impurity Profile
    • Chromatographic Purity
    • Isomer detection
  • General Procedure to read and understand different pharmacopoeia HPLC analytical method
  • Calculation of mobile phase required for analysis
  • Mobile phase preparation as per STP
  • Precaution during weighing of solid and liquid material for HPLC analysis
  • Sample and standard solution preparation as per STP
  • System suitability parameters achievement during analysis
  • HPLC parameter adjustment criteria as per different pharmacopoeia like USP BP to achieve system suitability parameters
  • Method Preparation
  • Batch Preparation
  • General injection criteria for different analysis like RS, Assay etc
  • Integration of Chromatograms of different analysis
  • Interpretation of HPLC chromatograms
  • Reporting of result of HPLC analysis
  • To report the result understanding the different terms and the language of pharmacopoeia
  • HPLC method development
    • Basics of HPLC Method Development
      • What goals do I have for the method?
      • Common Mistakes in Method Development
  • HPLC Method Development – A Proposed Procedure
    • At Your Desk
    • In the Laboratory
  • Choosing the Appropriate HPLC Column
    • Choice of Column Bonded Phase Characteristics
    • Choice of Column Particle Physical Characteristics
    • Column Selection Chart
    • Choosing the Bonded Phase
    • Functional Group Polarity Comparisons
    • Choosing the Particle Physical Characteristics
    • Column Dimensions
    • Particle Shape
    • Particle Size
    • Surface Area
    • Pore Size
    • Bonding Type
    • Carbon Load
    • End capping
    • Selection of Mobile Phase
    • Selection of Diluents
    • Selection of wavelength
    • Selection of Flow rate
  • HPLC method Validation
    • HPLC Method Validation
    • Introduction
    • Method Validation Vs Verification
    • Guideline for method validation
    • Parameters and Tests for Method Validation
      • Specificity
      • Selectivity
      • Precession
        • Repeatability
        • Intermediate precision
        • Reproducibility
      • Accuracy
      • Linearity
      • Range
      • Limit Of Detection
      • Limit of Quantitation
      • Ruggedness
      • Robustness