HPLC Instrumentation Training

HPLC Instrumentation Training

 

The HPLC Instrumentation Training course comprises of the following modules:

  • Introduction of HPLC
  • Parts of HPLC instrument
  • Solvent reservoirs
  • HPLC Solvent Pumping Systems
    • Type of pump
  • Auto samplers
    • Looping of sample and standard solution
  • HPLC Column
    • Types of column
    • Column Chemistry
    • Selection of column for analysis
  • Detectors
    • Types of Detector and its use for different types of analysis
  • Mode of HPLC analysis
    • Reverse phase (partition) chromatograph
    • Ion-Pair Chromatography
    • Normal phase (absorption) chromatography
  • Elution Mode In HPLC analysis
    • Isocratic mode
    • Gradient mode
  • Integration and operational software
    • Basic features of HPLC software

 

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